Closed - HICCC (Cancer Center) Epigenetics

Overview of Services

Epigenetics Service

The epigenetics shared resource provides DNA methylation analysis through targeted sequencing. The technology that is currently in place uses the Fluidigm Access Array system for targeted Next Generation Sequencing (NGS) of bisulfite treated DNA on the Illumina MiSEQ platform. Whole genome bisulfite sequencing on HiSeq is also available on a more limited first-come-first serve basis. The epigenetics service also provides technical assistance on custom design of their projects, as well as advice on outsourcing of whole genome methylation by Illumina Infinium 450K methylation arrays and deep bisulfite sequencing. This service has been in operation since January 2013.

Services include:

• Bisulfite treatment of DNA samples
• Targeted bisulfite PCR primer design
• Fluidigm 48x48 targeted bis-PCR and sequencing sample preparation
• MiSeq sequencing of bisulfite treated targeted sequencing libraries
• Analysis of the bis-seq data
• HiSeq whole genome bis-seq on a limited basis
• Advice and help with project design and, where necessary, outsourcing for large-scale projects

Before using the service, read the Sample Submission Instructions. Please note that DNA should not be dropped off unless previously arranged with the facility. Researchers are encouraged to initiate discussions regarding their project plans with the service staff in order to best facilitate proper DNA sample preparation, target region details, and the pipeline of data generation.

Leadership

Location and hours of operation

Links and Resources

Methylation Primer Design

Bioinformatics Site for Methylation Analysis      

Sequence Alignment Tool

Fluidigm Website

Illumina MiSeq Desktop Sequencer Website

Contacts