Microbiology & Immunology Core Facility

Overview of Services

 

The Department of Microbiology & Immunology Flow Cytometry Core assists researchers in flow cytometry based studies. Cell samples can be quickly analyzed based on phenotypic markers and functional assays. Characterization of distinct cell populations based on these techniques has become increasingly important in biomedical research. Flow cytometry is especially useful for physically separating specific sub-populations defined by specific parameters using a fluorescence activated cell sorter (FACS). The microscope core provides researchers the ability to obtain high quality imaging data using state-of-the-art microscopy instruments. 

 

Services include:

  • Analytical flow cytometry for immunophenotyping
  • Flow cytometry for apoptosis and cell cycle
  • Fluorescence-activated cell sorting (FACS)
  • Fluorochrome panel design

The shared resource operates three flow cytometers. The LSRFortessa and LSR II are designed for multi-fluorescence analysis while the custom configured FACS Aria II SORP high-speed cell sorter is capable of rapid sorting of cells, bacteria, yeast, and other small particles based on multiple parameter characteristics into highly pure populations. After September 19, 2017 we will have successfully standardized our two flow cytometers and cell soter with five lasers capable of simultaneous 18-color detection (i.e. 488nm - 2 PMTs, 405nm - 7 PMTs, 355nm - 3 PMTs, 640nm - 3 PMTs, and 561nm - 3 PMTs) of apoptosis, DNA/cell cycle analysis, detection of novel living color fluorescent proteins (DsRed, mCherry, tdTomato, mOrange, mPlum, et al.), immunophenotyping and calcium influx experiments.

 

The flow cytometric separation of cells from unfixed animal tissue is possible on the BD FACSAria cell sorter, which is housed in a Baker BioProtect IV biohazard cabinet. For unfixed human specimens, please contact Amir Figueroa. Before the initial sort is performed, each user is required to provide biosafety information including cell type, origin of sample and special requirements.

 

In additional to these systems, the facility also has a state-of-the-art Zeiss LSM710 ConfoCorr confocal imaging microscope system, a Zeiss AxioVert microscope, and a Typhoon Trio imaging system.

 

Leadership

Amir Figueroa
Manager
Phone: 212-342-4089
Email: af2615@cumc.columbia.edu

 

James Lapin
Administrator
Phone: 212-305-4046
Email: jl2787@cumc.columbia.edu

 

Carol Duigou
Accounts Manager
Phone: 212-305-3647
Email: ced4@cumc.columbia.edu

Location and hours of operation

Hours Location

 

Staffed 
Monday through Friday 9 AM to 5 PM

Trained Users
24/7 access through a swipe card system.

 

Department of Microbiology & Immunology
Flow Cytometry Core
Columbia University
701 W. 168 St., HHSC 1211A & 1201
New York, NY 10032

Links and Resources

htttp://www.microbiology.columbia.edu/fcc

Contacts

Name Role Phone Email Location
Amir Figueroa
Manager
 
212-342-4089
 
af2615@cumc.columbia.edu
 
HHSC 1211-A
 

Services

Name Description