Flow Cytometry, Microscopy, and Shared Equipment (CCTI/HICCC)

Flow Cytometry

The Flow Cytometry Core of the Columbia Center for Translational Immunology (CCTI) and Herbert Irving Comprehensive Cancer Center (HICCC) provides training and access to state-of-the-art flow cytometry for biomedical investigators at the Columbia University Medical Center. Among the many documented applications, characterization and quantification of cells for surface protein, cytokine, intracellular transcription factor and signalingmolecules are the most common experiments performed in the flow cytometry core facility. The CCTI/HICCC Flow Core includes seven analytic flow cytometers and four flow sorters.


The analytic flow cytometers include the BD FACSCantoII, BD Fortessa, BD LSRII which acquire data up to 10, 16, 21 parameters, respectively. While CantoII and Fortessa meet the needs of many basic experimental needs, our LSRII is among the most advanced cytometers. The LSRII harbors 6 lasers (355, 405, 488, 532, 594 and 633nm), which are able to excite a large fraction of commercially available fluorochromes (colors). In addition to the wide spectrum of lasers, the LSRII is equipped with the 96-well plate reader which allows high throughput analysis of 96 samples in short duration. This high throughput capacity makes projects such as tissue mapping and drug screening possible.


The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora system is highly flexible, intuitive, and ultra-sensitive. Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor detector arrays enables more efficient spectrum capture for dyes emitting in the 365-829 nm range. CWDM full spectrum technology unlocks each fluorochrome to express themselves fully across the entire light spectrum. Flat-top beam profiles, combined with a uniquely designed fluidics system, translate to outstanding performance at high sample flow rates. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity. The Aurora system suits every laboratory’s needs from simple to high complexity applications.


The 4-laser BD Influx cell sorter can sort on 17 parameters and provide 4 populations of cells. Importantly, the BD Influx meets BSL 2 biosafety specifications for viable human cell sorting. With the BD influx cell sorter, the populations of interest can be isolated for downstream experiments such as cell expansion/functional studies and DNA/RNA characterization. Please contact the facility staff for any issues regarding training, assistance and access with flow including protocol design, instrument operation, data analysis, and troubleshooting.



Caisheng Lu (Luke)

Tel: (212) 305-6596
Email: cl3055@cumc.columbia.edu

Ching-Yuan Chen (Steve)

Tel: 212-851-4576
Email: cc3538@cumc.columbia.edu

Our Webpage : CCTI/HICCC flow core


Location and hours of operation

Hours Location

Hours Staffed:

Monday-Friday 10:00am - 6:00pm

Hours Open: 24/7 


P&S, PS17-514, PS17-503
630 West 168th Street
New York, NY 10032


ICRC 301A, 325 and 326
1130 St. Nicholas Avenue
New York, NY 10032 


Links and Resources

CCTI flow core


Shared Equipment Core

We have the following for CCTI members and collaborators:

  • Cell freezer BB1734
  • Cytospin BB1511
  • Electroporator BB1511
  • Cryostat #1 BB1707
  • Sorvall Highspeed centrifuge BB1707
  • Ultracentrifuge BB1527
  • Elisa reader PS17-503
  • Biotek Plate reader PS17-503
  • Elispot machine PS17-503
  • ABII QOCR PS17-503
  • Animal irradiator RS2000 BB2001




Dr. Anette Wu - CCTI operation director
Phone: (212) 304-5695
Email: aw2342@cumc.columbia.edu


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