Welcome!
The Flow Cytometry Core of the Columbia Center for Translational Immunology (CCTI) provides training and access to state-of-the-art flow cytometry for biomedical investigators at the Columbia University Medical Center. Among the many documented applications, characterization and quantification of cells for surface protein, cytokine, intracellular transcription factor and signalingmolecules are the most common experiments performed in the flow cytometry core facility. The CCTI Flow Core includes three analytic flow cytometers and two flow sorters.
The analytic flow cytometers include the BD FACSCantoII, BD Fortessa, BD LSRII which acquire data up to 10, 16, 21 parameters, respectively. While CantoII and Fortessa meet the needs of many basic experimental needs, our LSRII is among the most advanced cytometers. The LSRII harbors 6 lasers (355, 405, 488, 532, 594 and 633nm), which are able to excite a large fraction of commercially available fluorochromes (colors). In addition to the wide spectrum of lasers, the LSRII is equipped with the 96-well plate reader which allows high throughput analysis of 96 samples in short duration. This high throughput capacity makes projects such as tissue mapping and drug screening possible.
The 4-laser BD Influx cell sorter can sort on 17 parameters and provide 4 populations of cells. Importantly, the BD Influx meets BSL 2 biosafety specifications for viable human cell sorting. With the BD influx cell sorter, the populations of interest can be isolated for downstream experiments such as cell expansion/functional studies and DNA/RNA characterization. Please contact the facility staff for any issues regarding training, assistance and access with flow including protocol design, instrument operation, data analysis, and troubleshooting.
Caisheng Lu
Tel: (212) 305-6596
Email: cl3055@cumc.columbia.edu
Wei Wang
Tel: 212-851-4950; 212-851-4603
Email: ww2351@cumc.columbia.edu
| Hours | Location |
|
Hours Staffed: Monday-Friday 10:00am - 6:00pm Hours Open: 24/7 |
P&S, PS17-514 P&S, PS17-503 |
Our in vivo multiphoton/confocal microscopy is advantageous in the following analyses:
Leica TCS SP8 has been designed for confocal microscopy with optimal photon efficiency and high speed. All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging. Backing up this sensitive detection are a high speed scanning system with up to 428 frames per second, large field of view of field number 22 and accelerated Z-stacking by a novel mode for the SuperZ galvanometer called Galvoflow. This suits the instrument for the most demanding samples, highest speed while offering full confocal resolution. Features include:
Joji Fujisaki
Phone: (212) 304-5695
Email: jf2819@cumc.columbia.edu
| Hours | Location |
| By appointment only |
Black Bldg, BB828 |
We have the following for CCTI members and collaborators:
Dr. Anette Wu - CCTI operation director
Phone: (212) 304-5695
Email: aw2342@cumc.columbia.edu
