Flow Cytometry (Columbia Stem Cell Initiative)

Overview of Services

CSCI Flow Cytometry was established as an integral component of the Columbia Stem Cell Initiative and is a full-service flow cytometry resource center that offers the Columbia University community and external users access to high-end flow cytometry and molecular biology instrumentation.

 

Our core facility is home to the following instruments: 

 

  • Two 20-parameter BD SORP FACSAria cell sorters
  • A fully-automated 6-parameter Bio-Rad S3e cell sorter
  • A 14-parameter BD FACSCelesta analyzer
  • A 30-parameter Bio-Rad ZE5 cell analyzer
  • A 12-parameter Luminex ImageStreamX MkII imaging cytometer
  • A Luminex-200 bead-based protein and nucleic acid multiplexed assay system
  • A Fluidigm BioMark HD microfluidic multiplexed genomic analyzer
  • An Applied Biosystems QuantiStudio 7 Flex real-time qPCR system

 

We offer both assisted and self-service sorting and a full training program for the flow cytometer analyzers and the flow cytometer sorters.

Additionally, CSCI Flow Cytometry offers technical assistance for experimental design, troubleshooting and data analysis. 

 

Please refer here for CSCI Flow Cytometry policies.

 

Panel Design: All users are encouraged to use Fluorofinder, an online panel building tool, to design experiments on our instruments. Please click here for our Fluorofinder page.


Leadership

Dr. Emmanuelle Passegué | Director

Email: ep2828@cumc.columbia.edu

 

Michael Kissner | Director of Operations                                         
Email: mdk2108@cumc.columbia.edu                     
Phone: 212-305-2561 (lab) 914-715-8765 (mobile)

 

Daniel Troast | Flow Cytometrist

Email: dt2638@cumc.columbia.edu

 

Location and hours of operation

Hours Open 
Location

24/7

Vagelos Physicians & Surgeons Building

630 W. 168thStreet

Room 11511

New York, NY 10032

 

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Contacts

Name Role Phone Email Location
Michael Kissner
Director of Operations
 
212-305-2561 (lab) 914-715-8765 (mobile)
 
mdk2108@cumc.columbia.edu
 
P&S 11511
 
Daniel Troast
Flow Cytometrist
 
212-305-2561 (lab)
 
dt2638@cumc.columbia.edu
 
P&S 11511
 

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