Zuckerman Institute: Cellular Imaging

Overview of Services

The Cellular Imaging platform aims to enhance discovery within the Zuckerman Institute by providing access to cutting edge imaging technology. Our goal is to offer a combination of both commercial platforms and not-yet commercialized technologies that enable innovative research centered around structural and functional imaging.

Available resources include:

  • Nikon inverted A1R confocal - galvo and resonant laser scanning confocal for live and fixed imaging in tissue and cells
  • Zeiss upright LSM 880 confocal with Airyscan
  • Zeiss upright LSM 710 confocal
  • W1-Yokogawa inverted spinning disk confocal - high-speed high-sensitivity confocal microscope optimized for imaging tissue sections and live samples
  • W1-SoRa-Yokogawa inverted spinning disk confocal - high-speed high-sensitivity confocal super-resolution microscope for rapid imaging at resolutions up to 120nm lateral resolution
  • Bruker Vutara 452 - single molecule localization instrument capable of imaging in tissue and cells
  • AZ100 Slide Scanner - high-througput fluorescence and brightfield slide scanning microscope, capacity for 200 slides, ~15 minutes / slide
  • LaVision Biotec Ultramicroscope II light sheet microscope - for imaging cleared samples, optimized for DBE based clearing
  • Thorlabs Bergamo II Multiphoton Microscope
  • High-performance image analysis workstations - including Aivia, Imaris, NIS-Elements, and custom workflows for ImageJ/Fiji

Example brain section imaged using the AZ100 slide scanner labelled with DAPI, GFP, tdTomato and Alexa Fluor 647. Imaged by Dr. Zirong Gu, Costa lab.

Example brain section imaged using the AZ100 slide scanner labelled with DAPI, GFP, tdTomato and Alexa Fluor 647.

Imaged by Dr. Zirong Gu, Costa lab.

Getting Started

Login or create an iLab account using your Columbia CUID 

Links and Resources

Cellular Imaging Internal Website

Leadership

Darcy Peterka | Director | 212-853-1124 | dp2403@columbia.edu | L3-007
Luke Hammond | Manager | 917-561-9845 | lh2881@columbia.edu | L3-007

Acknowledgement

It is important to properly acknowledge you use of the Cellular Imaging platform in your work and publications. Acknowledgement allows us to demonstrate the platform's contribution to research, helps us justify ongoing costs, and in securing future funding for new instruments and technology to further support your research. 

If your publication makes use of the platform for image acquisition, analysis, or support, please use a general statement in the acknowledgements section, such as below:

     "Imaging was performed with support from the Zuckerman Institute’s Cellular Imaging platform." or  "We would like to thank Zuckerman Institute's Cellular Imaging platform for instrument use and technical advice.
 
The UltraMicroscopeII light sheet microscope was funded by an NIH Shared Instrumentation Grant (S10). If this instrument has been used in your publication please remember to acknowledge this funding specifically. For example:
 
     "Imaging was performed with support from the Zuckerman Institute’s Cellular Imaging platform, and the National Institute of Health (NIH 1S10OD023587-01)."
 
Additionally, if Cellular Imaging staff have made significant contributions to your publication, they should be acknowledged in the acknowledgements or by co-authorship, depending on the level of intellectual contribution.

Location and hours of operation

Hours Location

 9AM-6PM
Monday-Friday
*these are staffed hours

 24/7 for trained users

3227 Broadway
JLG L3-007 
New York, NY. 10027

 

Contacts

Name Role Phone Email Location
Luke Hammond
Manager
 
917-561-9845
 
lh2881@columbia.edu
 
L3-007
 
Darcy Peterka
Director
 
212-853-1124
 
dp2403@columbia.edu
 
L3-007
 

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