Human Immune Monitoring (HICCC)

Services

Vectra (multiplex tissue imaging with spectral microscopy)

  • 8 markers + DAPI 
  • Standard human panels
    • Solid Tumor Immunology: CD4, CD8, CD20, FoxP3, CD68, panCK
    • Tumor Infiltrating Lymphocyte: CD4, CD8, CD20, FoxP3, CD45RO, panCK
  • Custom services

NanoString (rapid RNAseq, frozen tissue & FFPE compatible)

  • Standard "ready-to-run" samples
  • Full services

10x Genomics (single-cell RNAseq)

  • General gene expression profiling (scRNAseq)
  • Paired α/β scTCRseq/scBCRseq
  • high-parametric surface marker characterization (CITEseq)
  • ATAC + General gene expression (MultiomeSeq)
  • Hashing of cells
  • After-hour services and self-services avaialble

Spatial Transcriptomics (Frozen tissue & FFPE compatible)

  • High cellular resolution (1-10 cell resolution) on average per spot depending on tissue type
  • Protein co-detection (Combine whole transcriptomal spatial analysis with immunofluorescence protein detection)
  • Capture area of 6.5 x 6.5 mm or 11 x 11 mm
  • Diverse Sample compatability
  • Streamlined data analyis

CyTOF (high-parametric mass cytometry)

  • Simultaneous detection of >40 markers on same cells
  • Library available of >100 metal-tagged antibodies (both human and mice)
  • Separate and distinct signals, no autofluorescence, no FMOs required
  • Standard panels with customization available
  • Conjugating markers of your choice to incorporate in the panel
  • Barcoding of ~30 samples in one tube (Less Variable signal intensity)
  • FlowJo compatible analysis

Cytek Aurora (high-parametric spectral cytometry)

  • 25+ biological markers

MSD (cytokine analysis)

Leadership

Benjamin Izar, MD, PhD, Director

Parin Shah, PhD

Contact Us

  • Main Office: PS17-510 (630 W 168th St)
  • Cytometry & Single Cell Analysis: PS17-514 (630 W 168th St)
  • Multiplex Tissue Analysis: VC15-205 (622 W 168th St)

 

Links and Resources

Shared Resources

Contacts

Name Role Phone Email Location
Benjamin Izar
Faculty Director
 

 
bi2175@cumc.columbia.edu
 

 
Parin Shah
Manager
 

 
ps3280@cumc.columbia.edu
 
VP&S 17-510
 

Service list


Search available services: View: by category alphabetically
10x Chromium (8)
Nanostring Rxn (7)
Other (10)
Vectra (7)
Visium (2)

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