Human Immune Monitoring (CCTI)

Services

Vectra (multiplex tissue imaging with spectral microscopy)

  • 6 markers + DAPI + AF
  • Standard human panels
    • Solid Tumor Immunology: CD4, CD8, CD20, FoxP3, CD68, panCK
    • Tumor Infiltrating Lymphocyte: CD4, CD8, CD20, FoxP3, CD45RO, panCK
  • Custom services

NanoString (rapid RNAseq, frozen tissue & FFPE compatible)

  • Standard "ready-to-run" samples
  • Full services

10x Genomics (single-cell RNAseq)

  • General gene expression profiling (scRNAseq)
  • Paired α/β scTCRseq/scBCRseq
  • high-parametric surface marker characterization (CITEseq)
  • After-hour services and self-services avaialble

CyTOF (high-parametric mass cytometry)

  • 45+ biological markers on unique channels
  • ability to barcode 40+ samples in same staining and acquisition tube
  • no need for single-color controls

Cytek Aurora (high-parametric spectral cytometry)

  • 25+ biological markers

MSD (cytokine analysis)

iSpot (multicolor ELISpot and FluoroSpot)

Other shared equipment

  • TapeStation (automated DNA/RNA electrophoresis tool)

Leadership

Charles G. Drake, MD, PhD, Director

Yvonne Saenger, MD, Co-Director

Xinzheng Victor Guo, PhD, Manager

Contact Us

  • Main Office: PS17-510 (630 W 168th St)
  • Cytometry & Single Cell Analysis: PS17-514 (630 W 168th St)
  • Multiplex Tissue Analysis: VC15-205 (622 W 168th St)

 

Contacts

Name Role Phone Email Location
Xinzheng Victor Guo
Core Manager / Associate Research Scientist
 
212-304-7607
 
xg2295@columbia.edu
 
PS17-510
 
Xiang Wang
Technician: Tissue Specialist
 
212-305-3902
 
xyw1@cumc.columbia.edu
 
VC15-205
 
Yan Lu
Technician: Tissue Specialist
 

 

 
VC15-205
 
Yucui Zhu
Technician: Single-Cell Analysis
 

 
yz81@columbia.edu
 
PS17-514
 

Map