Zuckerman Institute: Flow Cytometry

Overview of Services

The flow cytometry platform enables researchers to identify and sort specific cells of interest, based on fluorescent labels and light scatter properties of the individual cells, at a rate of thousands per second. 

There are an ever widening array of fluorescent probes for cells including molecularly introduced fluorescent proteins to label specific gene activity, antibodies for cell surface and internal antigens, fluorescent markers for cell activation, viability, proliferation, and more.

The Flow Cytometry Core's range of capabilities include sorting up to 6 defined populations of cells simultaneously, filling your collection tubes with millions of cells or depositing a single cell into each well of a 384 well plate.

Leadership

Director of Flow Cytometry and Senior Science Officer - Ira Schieren - is104@columbia.edu

Manager of Flow Cytometry - Max Wallach - mw3793@columbia.edu

Location and hours of operation

 Links and Resources

Cell Sorting Advice: How to prepare cells for cell sorting   --   Use DAPI to eliminate dead cells from analysis   --   Cell Clump Elimination w. DNAse   --   Cell Clump Elimination w Cell Strainer    --   10 things to know about cell sorting

Other Links and Resources: BD Introduction to Flow Cytometry (Modules)   --    What is Flow Cytometry?   --   BioRad Flow Cytometry Protocols   --   Florofinder Panel Builder (Intitute; Columbia. Lab; Zuckerman Institute-Flow Cytometry for Sony, HHMI-Neuroscience for Astrios)      

Please contact Max Wallach mw3793@columbia.edu to discuss your flow cytometry needs.

Contacts